| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Roseobacter denitrificans | TCGAGCGTTCGCAAATGC | ACCCGACAAACGCACTGT | 59.53 | 59.81 | 194 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Roseobacter denitrificans | TACCGCATCACACTGGCTG | AGCACAAAACTGCGCTGG | 60.08 | 59.28 | 164 | 141 |
100.00%
|
100.00%
|
| Primer 3 | Roseobacter denitrificans | TTCAGGAAAGCGGACCACC | TTGCCAAAAGCTGCGCTG | 59.93 | 59.97 | 246 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Roseobacter denitrificans | TGCCTTTGTGGTGTCGCA | CATCCACTTCGGCTTCGGA | 60.12 | 59.78 | 161 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Roseobacter denitrificans | TGCCTTTGTGGTGTCGCA | ATCCACTTCGGCTTCGGAC | 60.12 | 59.78 | 160 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Roseobacter denitrificans | TTGCTGGTTCGGATCGCA | AGTTCGTGACCATGCGCA | 59.65 | 59.97 | 295 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Roseobacter denitrificans | TTCCTCGAAGGGTTGCTGC | TGTCGCCATTGTCCAGCA | 60.30 | 59.57 | 151 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Roseobacter denitrificans | TGGCAACGCTATCCGCAT | TCTGATCGAGCAAGGCGTC | 59.81 | 59.86 | 217 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Roseobacter denitrificans | TCAAAGCCAGCTGGTGCA | TCGCCTTTCAGCACCACA | 59.80 | 59.49 | 187 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Roseobacter denitrificans | TCAAAGCCAGCTGGTGCA | ATCGCCTTTCAGCACCACA | 59.80 | 59.93 | 188 | 137 |
100.00%
|
100.00%
|